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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been noticed in lots of tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was discovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential function in promoting mobile-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have been explained. Two extra splice variants were being documented but comprehensive nucleotide sequence information and facts is just not however readily available.
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Blocking peptides are peptides that bind exclusively to the focus on antibody and block antibody binding. These peptide commonly has the epitope recognized through the antibody.
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This guidebook outlines even more achievable optimization of cellular and immunoassay parameters to be sure the best possible success are...
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This guide outlines additional achievable optimization of cellular and immunoassay parameters to make certain the absolute best benefits are...
Antibodies certain to the blocking peptide no more bind on the epitope about the goal protein. By comparing the staining from your blocked antibody compared to the antibody on your own, one can see which staining is unique.
Target Details Cyclin E belongs for the very conserved cyclin family members, whose members are characterised by a extraordinary periodicity in protein abundance with the mobile cycle. Cyclins perform as regulators of CDK kinases. Distinctive cyclins exhibit distinct expression and degradation styles which add for the temporal coordination of every mitotic celebration. Cyclin E kinds a complex with and capabilities like a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress through S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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